The Influence of Oregano Essential Oil and Rhus Coriaria L. on Qualitative Parameters and Microbiological Indicators of Hens Eggs Content

Henrieta Arpášová, Miroslava Kačániová, Branislav Gálik, Martin Mellen


In this experiment the effects of supplementation of the diet for laying hens with oregano essential oil and Rhus coriaria L.seeds addition on physical and microbiological egg parameters were studied. Hens of laying hybrid Hy-Line Brown (n=30) were randomly divided into 3 groups (n=10) and fed for 20 weeks with diets with oregano essential oil and Rhus coriaria L.seeds supplemented. In the control group hens received feed mixture with no additions. The diets in the first experimental groups were supplemented with 1 ml/kg oregano essential oil. The feed for second experimental group of birds consisted of basal diet supplemented with Rhus coriaria L. seeds (1% in total feed mixture). The results suggest that the most of qualitative parameters of egg internal content and egg shell were not significantly influenced with oregano oil or Rhus coriaria L. seeds addition (P>0.05). A statistically significant difference in favour of the experimental groups compared with the control group was observed in two indicators of albumen quality, in the index of albumen and in the Haugh Units (P<0.05). The highest total number of bacteria and count of coli forms bacteria was found in the control group. The number of lactobacilli was zero in all groups.


oregano essential oil, Rhus coriaria L. seeds, table eggs quality, microbiological quality

Full Text:



NOSTRO, A. - ROCCARO, A. S. - BISIGNANO, G. - MARINO, A. - CANNATELLI, M. A. - PIZZIMENTI, F. C. - CIONI, P. L. - PROCOPIO, F. - BLANCO, A. R. 2007. Effcts of oregano, carvacrol and thymol on Staphylococcus aureaus and Staphylococcus epidermidis biofilms. In Journal of Medical Microbiology, vol. 56, 2007, p. 519-523.

VIUDA-MARTOS, M. - RUIZ-NAVAJAS, Y. - FERNÁNDEZ-LÓPEZ, J. - PÉREZ-ÁLVAREZ, J. A. 2007. Antifungal activities of thyme, clove and oregano essential oils. In Journal of Food Safety, vol. 27, 2007, p. 91–101.

GUTIERREZ, J. - BOURKE, P. – LONCHAMP, J. - BARRY-RYAN, C. 2009. Impact of plant essential oils on microbiological, organoleptic and quality markers of minimally processed vegetables, In Innovative Food Science & Emerging Technologies, vol. 10, 2009, p. 195–202.

SALGUEIRO, L. R. - CAVALEIRO, C. - PINTO, E. et al. 2003. Chemical composition and antifungal activity of the essential oil of Origanum virens on Candida species. In Planta Medica, vol. 69, 2003, no. 9, p. 871-874

BAYDAR ET, A. L. - BAYDAR, H. - SAĞDIÇ, O. – ÖZKAN, O. - KARADOĞAN, T. 2004. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. In Food Control, vol. 15, 2004, p. 169–72.

KAČÁNIOVÁ, M., NOVÁKOVÁ, I., HAŠČÍK, P. 2011. Význam probiotík a ich význam na mikroflóru gastrointestinálneho traktu hydiny. Nitra : SPU, 181 s. ISBN 978-80-552-0655-4

ČUBOŇ, J. - HAŠČÍK, P. - KAČÁNIOVÁ, M. - ARPÁŠOVÁ, H. - PRÍVARA, Š. 2009. Vplyv podávania biologicky účinných látok na technologické a nutričné vlastnosti vybraných produktov živočíšneho pôvodu. Nitra : SPU, 2009. 116 s. ISBN 978-80-552-0291-4.

ARPÁŠOVÁ, H. 2011. Fytobiotiká ako náhrada antibiotických stimulátorov rastu a ich vplyv na úžitkovosť a kvalitu vajec sliepok znáškového typu. Nitra : SPU, 2011. 101 s. ISBN 978-80-552-0555-7.

ZALACAIN et al., 2003. Optimalisation of extraction and identification of gallotannins from sumac leaves. In Biosys. Eng. vol. 84, 2003, no. 2, pp. 211-216

PAVELKOVÁ, A. 2012. Intelligent packaging as device for monitoring of risk factors in food. In Journal of microbiology, biotechnology and food sciences, vol. 2, 2012, no. 1, p. 282-292.

DAWSON, R. C. - COX, J. M. - ALMOND, A. - MOSES, A. 2001. Food Safety Risk Management in Different Egg production System. Kingstone : Rural Industries Research and Development Corporation, 50 p., ISBN 0 6242 58333 1.

FLOROU-PANERI, P. - NIKOLAKAKIS, I.. - GIANNENAS, I. - KOIDIS, A. - BOTSOGLOU, E. - DOTAS, V. MITSOPOULOS, I.. 2005. Hen performance and egg quality as affected by dietary oregano essential oil and tocopheryl acetate supplementation. In International Journal of Poultry Science [online]. vol. 4, 2005, no. 7, p. 449-454.

GÜNDÜZ, G. T. – GÖNÜL, S. A. – KARAPINAR, M. 2010. Efficacy of sumac and oregano in the inactivation of Salmonella typhimurium on tomatoes. In International Journal of Food Microbiology, vol. 141, 2010, no. 1-2, p. 39-44.

SENKÖYLÜ, N. - AKYÜREK, H - SAMLI, H. E. - YURDAKURBAN, N. 2004. Performance and egg weight of laying hens fed on the diets with various by- product oils from the oilseed extraction refinery. In Pakistan Journal of Nutrition, vol. 3, 2004, no. 1, p. 38-42.

YANG, C. J. - UUGANBAYAR, D. - SUN, S. S. - FIRMAN, J. D. 2003. Effect of dietary green tea on productivity and egg composition in laying hens. In: Animal Science Journal, vol. 81, 2003, p. 204.

CANOGULLARI, S. - KARAMAN, M. - ERDOGAN, Z. - BAYLAN, M. - KUCUKGUL, A. - DUZGUNER, V. - KEMALI OZUGUR, A. 2009. Effect of garlic powder on egg yolk and serum cholesterol and performance of laying hens. In Bulletin of the Veterinary Institute in Pulawy, vol.53, 2009, no. 3, p. 515-519.

BOZKURT, M. - KUCUKYILMAZ, K. – CATLI, A.U. – CINAR, M. – BINTAS, E. – COVEN, F. 2012. Performance, egg quality, and immune response of laying hens fed diets supplemented with mannan-oligosaccharide or an essential oil mixture under moderate and hot environmental condi. In Poultry Science, vol. 91, 2012, no. 6, p. 1379-1386.

YALCIN, S. - ONBASILAR, E. E. - REISLI, Z. - YALCIN, S. 2006: Effect of garlic powder on the performance, egg traits and blood parameters of laying hens. In Journal of the Science of Food and Agriculture, vol. 86, 2006, no. 9, p. 1336–1339. ISSN: 0022-5142.

RADWAN NADIA, L. - HASSAN, R. A. - QOTA, E. M. - FAYEK, H. M. 2008. Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. In International Journal of Poultry Science, vol. 7, 2008, no. 2, p. 134-150.

YANG, C. J. - UUGANBAYAR, D. - SUN, S. S. - FIRMAN, J. D. 2003. Effect of dietary green tea on productivity and egg composition in laying hens. In: Animal Science Journal, vol. 81, 2003, p. 204.

YODSERANNE, R. – CHANTSAVANG, S. – BUNCHASUK, C. 2003. Effect of dietary supplementation of mixed culture compost, plantand animal bioextract compost on layer´s productive performance and egg quality. In Animal and Veterinary Medicine, 2003, p. 119–126.

BOTSOGLOU, N. - FLOROU-PANERI, P. - BOTSOGLOU, E. - DOTAS, V. - GIANNENAS, I. - KOIDIS, A. - MITRAKOS, P. 2005. The effect of feeding rosemary, oregano, saffron and alpha-tocopheryl acetate on hen performance and oxidative stability of eggs. In South African Journal of Animal Science, vol.35, 2005, no. 3, p. 143-151.

AYERZA, R. - COATES, W. 2002. Dietary levels of chia: influence on hen weight, egg production and sensory quality, for two strains of hens. In British of Poultry Science. ,2002, no. 2, p. 283-290.

AYDIN, R. - BAL, A. M. - OZUGUR, K. A. - TOPRAK, C. H. H. - KAMALAK, A. - KARAMAN, M. 2006. Effects of black ssed (Nigella sativa L.) supplementation on feed efficiency, egg yield parameters and Shell Quality in Chickens. In Pakistan Journal of Biological Sciences, vol. 9, 2006, no. 2, p. 243-247.

SAHINLER, S. - SAHIN, A. - GORGULU, O. 2005. Ferula eleaochytris powder effect in layer diet on feed intake and some egg parameters using a multivariate analysis method for repeated measures, In Journal of Applied Animal Research, vol. 27, 2005, no. 1, p. 29-33.


  • There are currently no refbacks.

Copyright (c)

Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at]
Phone: +40-256-277160, Fax.: +40-256-277110