Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambs

Cristina Lazar, Alexandru Mihail Gras, Mircea Catalin Rotar, Gina Cecilia Pistol, Rodica Stefania Pelmus, Elena Ghita


The objective of this study was to identify Myostatin (MSTN) gene polymorphism using PCR RFLP technique in order to improve sheep meat evaluation. There are data which show that MSTN is an inhibitor of skeletal muscle growth and a mutation in gene leads to increased muscle mass. Therefore, it is considered as an appropriate candidate gene for carcass meat quality. Blood samples were collected from 105 Teleorman Black Head (TBH) lambs and DNA was extracted using, Wizard Genomic DNA Purification Kit. Polymorphism of MSTN was determined by PCR amplification followed by RFLP method using restriction enzyme Hae III. Based on results, two genotypes mm and Mm were identified. It was identified homozygous genotype mm which not carry this mutation with 16.67% and heterozygous genotype Mm with 83.33%. M allele frequency was 42% and for allele m was 58%. Observed and expected values of MSTN genotypes were found in Hardy Weinberg disequilibrium, after χ2 test was calculated. MM individuals who carry this mutation were not identified in the analysed herd. This may be due to an empirical selection of this breed leading to absence of this genotype. That is why further investigation on a high number of animals are necessary to discover this mutation correlated with meat quality in TBH sheep.


myostatin; carcass; sheep; meat; evaluation; quality; polymorphism

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Balteanu V. A., Vlaic A., Pop F. D., Carsai T. C., Pascal C., Creanga St., Zaharia N., Padeanu I., Voia O. S., Sauer M., Sauer I. V., The study of αS1-casein genetic marker polymorphism in Carpathian goat breed, Scientific paper Animal Husbandry, Iasi, 2010, 53, 321-325

Balteanu I. V. A., Teza de doctorat, Studiul polimorfismelor genetice ale proteinelor majore din lapte la principalele rase de taurine, bubaline, ovine si caprine din Romania in scopul utilizarii lor ca markeri genetici in ameliorare si trasabilitate, 2011, USAMV Cluj Napoca, Facultatea de Zootehnie si Biotehnologii

Lazăr C., Pelmuş R., Marin D., Ghiţă E., Preliminary investigations on milk proteins polymorphism in Carpathian goats and in F1 Carpathian Saanen hybrids, Archiva Zootechnica, 2010, 13:4, 45-57

Schibler L., Vaiman D., Oustry A., Giraud-Delville C., Cribiu E.P., Comparative gene mapping: a fine-scale survey of chromosome rearrangements between ruminants and humans, 1998, Genome Res., 8, 901-915

Pinton P., Schibler L., Cribiu E., Gellin J., Yerle M., Localization of 113 anchor loci in pigs: improvement of the comparative map for humans, pigs, and goats, 2000, Mammalian Genome, 11, 4, 306-315

Supakorn C., The Important Candidate Genes in Goats - A Review, 2009, Walailak Journal Scientific Technology, 6, 1,17-36

Zhang C., Liu Y., Xu D., Wen Q., Li X., Zhang W., Yang L., Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance, 2012, Molecular Biology Rep., 39, 3, 3081-3087

Miranda M. E., Amigues Y., Boscher M. Y., Menissier F., Cortes O., Dunner S., Simultaneous genotyping to detect myostatin gene polymorphism in beef cattle breeds, Journal Animal Breeding and Genetics, 2002, 119, 361-366

Jiang Y.L., Fan X.Z., Xiao L.R., Xian X.X., Hu L.X., Du L.X., Wu C.X., Association of T-A mutation of myostatin gene with birth weight in Yorkshire pigs, 2002, Asian Australian Journal Animal, 20, 1342-1348

Gu Z.L., Zhang H.F., Zhu D.H., Li H., Single nucleotide polymorphism analysis of the chicken Myostatin gene in different chicken lines, 2002, Acta Genet. Sin., 29, 599-632

Soufy B., Mohamad Abadi, Shojaeian K., Baghizadeh A., Ferasaty S., Askari N., Dayani O., Evaluation of myostatin gene polymorphism in Sanjabi sheep by PCR-RFLP method, 2009, Animal Science Researches, 19, 1, 81-89.

Dehnavi E., Azari M. A., Hasani S., Nassiry M. R., Mohajer M., Ahmadi A. K., Shahmohamadi L., and Soheil Y., Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with YearlingWeight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep, Hindawi Publishing Corporation, Biotechnology Research International 2012, Article ID 472307, 5 pages doi:10.1155/2012/472307

Ansary M., Tahmoorespour M., Valeh M. V., Nassiry M. R., and Shahroudi F. E., Investigation of polymorphism of GDF-8 gene and its association with average daily gain in Baluchi sheep, in Proceedings of the 3th Congress on Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran, 2008.

Masoudi A., Hemrani H., Abbasi A. et al., “Using PCR-SSCP techniques to study polymorphism of myostatin gene and its association with production traits in Baluchi sheep, in Proceedings of the 4th National Biotechnology Congress of Iran, Kerman, Iran, August 2005.

Sorour J., Shahramnane K., Majid G., Study polymorphism myostatin gene in Mehraban’s sheep using PCR-RFLP method, Scientific International Lahore, 2014, 26, 3, 1129-1135

Georgieva S., Hristova D., Dimitrova I., Stancheva N., Sakova M. B., Molecular analysis of ovine calpastatin (CAST) and myostatin (MSTN) genes in Synthetic Population Bulgarian Milk sheep using PCR-RFLP, 2015, Journal BioSci. Biotechnology, 4, 1, 95-99

Azari M. A., Dehnavi E., Yousefi S., Shahmohamadi L., Polymorphism of calpastatin, calpain and myostatin genes in native Dalagh sheep in Iran, 2012, Slovak Journal Animal Science, 45, 1, 1-


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