Evaluation of enzymatic potentialities of Bacillus subtilis using as substrate different animal raw materials feed

Mihaela Dumitru, Lefter Nicoleta, Idriceanu Lavinia, Habeanu Mihaela

Abstract


In the present investigation, the production of amylase, cellulase, and protease activity by Bacillus subtilis ATCC 6051a was evaluated on different raw materials (soybean meal, peas, sorghum flour, corn) and combined feed (CF). The effect of various fermentation conditions (24-72 h) on enzymatic production through shake-flask culture (Erlenmeyer 100 mL) in optimum conditions (150 rpm, pH 7.0±0.2, 37◦C) was investigated. The inoculum strain presents 1.907 optical density (OD) 600 nm with a concentration of 1.6 x 109 CFU/mL. The screening showed a capacity of amylase, cellulase, and protease strain production. The maximum amylase level was obtained at 72 h when the strain was cultured in CF fermentation medium (13.19±0.15 U/mL), followed by corn (11.72±0.15 U/mL), peas (9.22±0.11 U/mL), soybean meal (7.29±0.19 U/mL) and sorghum (6.31±0.2 U/mL). For production of cellulase by DNS method, the activity was noticed in corn (4.39±0.04 U/mL), sorghum (4.05±0.02 U/mL), peas (2.94±0.06 U/mL), soybean meal (2.87±0.04 U/mL) and CF medium (1.41±0.02 U/mL). Maximum protease activity was observed after 24 h in CF (4.91±0.08 U/mL), with minimum enzyme production in the rest of fermentation media, both at 48 h and 72 h. In conclusion, qualitative analysis revealed that Bacillus subtilis ATCC 6051a could be successfully used for high scale production of amylase, cellulase and to less extend protease, particularly in fermented medium containing CF or corn, and may be considered a potential candidate for supplementation of animal feed. 


Keywords


enzymatic activity, shake flask fermentation, probiotic

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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