The effect of GnRH and PGF2α administration on estrus onset and dairy buffaloes conception rate

Remus Chiorean


The aim of this study was to investigate the effect of GnRH and PGF2α administration on the manifestation of estrus and on the conception rate in Romanian buffalo dairy buffaloes. Twenty-eight dairy buffaloes from the Șercaia Buffalo Breeding Research and Development Station, aged between 5 and 12, in the postpartum period, were divided into four batches of seven heads each, as follows: Lot I (between 25 and 30 postpartum days), Lot II (40 to 45 days postpartum), Lot III (55 to 60 days postpartum) and Lot IV (75 days postpartum). Dairy buffaloes were given intramuscularly GnRH (2.5 ml Receptal) on day 0, followed by intramuscular injection of PGF2α (2.5 ml Alfabedyl) on day 7 after GnRH injection. Estrus was observed 24-48 hours after PGF2α injection. Artificial insemination was performed 12-18 hours after visible signs of estrus. The results of the study showed that there were no differences (p> 0.05) between estrus treatments (100%), estrus onset (35.8 to 36.4 hours), estrus duration (17 to 18 hours) and conception rate (100%). In conclusion, in Romanian buffalo dairy buffaloes, estrus can be synchronized from 25-30 days after calving using a combination of GnRH and PGF2α.


Romanian buffalo; postpartum; dairy buffaloes; estrus; calving


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