GENETICAL INACTIVATION OF PIKEPERCH (SANDER LUCIOPERCA) SPERM USING UV IRRADIATION
Keywords:
pikeperch, sperm, genetical inactivation, UVAbstract
Although pikeperch is a promising species for intensive aquaculture and some
experiments regarding all-female production in this species were made, there are no
specifically data regarding genetical inactivation of pikeperch sperm. The aim of
this study is to test two different UV lights (15 and 30 Watts) and exposure times of
the diluted milt at UV irradiation for sperm genetical inactivation, in order to
establish the first step in the gynogenesis protocol for pikeperch. The milt collected
from 4 clinically health adult pikepearch males (3-4 years old) was diluted 1:9 with
Ringer solution, after that being placed into Petri dishes (Ø = 3 cm) in a thin layer
of 1 mm. Two UV lights (λ = 253.7 nm) at 15 Watts (variant V1) and 30 Watts
(variant V2) were used in our experiments. These were placed 20 cm above Petri
dishes, and were established for each variant, 5 different periods for exposure to the
irradiation: 8 min. (T1), 11 min. (T2), 14 min. (T3), 17 min. (T4) and 20 min. (T5). The
successful of irradiation was assessed by percent of fertilization (at 2 hours post
fertilization), daily percent of survival, percent of hatching and percent of haploids
(embryos/larvae with severely curved backbones and other deformities). After this
study we could conclude that genetically inactivation of the pikeperch sperm can be
performed with 15 Watts and 30 Watts UV lights at an exposure time which can vary
between 8 and 20 minutes.
References
Demska-Zakes K., Zakes Z., 1997, Effect of 17α-methyltestosteroneone on
gonadal differentiation in pikeperch, Stizostedion lucioperca, Aquaculture
Research, 28, 59-63.
Denska-Zakes K., Zakes Z., 2008, Endocrine sex control strategies of
pikeperch (Sander lucioperca), in Fontaine P. et al, Percid Fish Culture –
From Research to Production. Abstracts and short communications.
Dunham R.A., 2005, Aquaculture and Fisheries Biotechnology. Genetic
Approaches, CABI Publishing, Cambrige, MA.
Felip, A., Zanuy, S., Carrillo, M., Piferrer, F., 2001, Induction of
triploidy and gynogenesis in teleost fish with emphasis on marine
species. Genetica, 111, 175-195
Grozea A., Muscalu-Nagy C., Banatean-Dunea I., 2006, Chromosomes
inactivation in common carp spermatozoa in order to obtain all-female
population by means of gynogenesis, Proceeding Abstr., „Animal Husbandry,
Veterinary and Agroeconomy in transitional processes”, University of Novi
Sad, Herceg Novi, p. 88.
Hilge V., Steffens W., 1996, Aquaculture of fry and fingerling of pike-perch
(Stizostedion lucioperca L.) – a short review, Journal of applied Ichthyology,
, 167-170.
Kucharczyk D., Kestemont P., Mamcarz A., (Ed.), 2007, Artificial
reproduction of pikeperch, Mercurius Kaczmarek Andrzej, Olsztyn.
Morgan, A.J., Murashige, R., Woolridge, C.A., Luckenbach, J.A.,
Watanabe, W.O., Borski, R.J., Godwin, J., Daniels, H.V., 2006,
Effective UV dose and pressure shock for induction of meiotic
gynogenesis in southern flounder (Paralichthys lethostigma) using black
sea bass (Centropristis striata) sperm, Aquaculture, 259, 290-299.
Moyle P.B., Cech Jr. J.J., 2000, Fishes. An Introduction to Ichthyology 4th ed.,
Prentice-Hall Inc., NJ.
Rhanga M.M., Shammi Q.J., 2002, Fish Biotechnology, Ed. Agrobios,
Jodhpur.
Strüssmann C.A., Karube M., Miranda L.A., Patino R., Somoza G.M., Uchida
D., Yamashita M., 2005, Methods of Sex Control in Fishes and an Overview
of Novel Hypotheses concerning the Mechanisms of Sex Differentiation in
Pandian T.J. et al. editors, Fish Genetics and Aquaculture Biotechnology,
Science Publishers Inc, Enfield, NH.
