Antimicrobial Activity of Crude Methanolic Extracts from Ganoderma lucidum and Trametes versicolor

Authors

  • Lukáš Hleba Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. Andreja Hlinku 2, 949 76 Nitra, Slovakia.
  • Nenad Vuković University of Kragujevac, Faculty of Science, Department of Chemistry, Kragujevac, Serbia.
  • Jana Petrová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. Andreja Hlinku 2, 949 76 Nitra, Slovakia.
  • Miroslava Kačániová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. Andreja Hlinku 2, 949 76 Nitra, Slovakia.

Keywords:

Antimicrobial activity, macromycetes methanolic extracts, MIC, Ganoderma lucidum, Trametes versicolor

Abstract

In this paper the antimicrobial activity of crude methanolic extracts obtained from Ganoderma lucidum and Trametes versicolor were investigated. The antimicrobial activities of the extracts against E. coli, P. aeruginosa, S. epidermis, E. raffinosus, S. cerevisiae and C. albicans were determined by the microbroth dillution method according by EUCAST in 96-well microplates. Microorganisms were obtained from Czech Collection of Microorganisms. Absorbances after and before the experiment were subtracted, converted to binary system and obtained values to Probit analysis were used. Of the two macromycetes extracts tested, not all extracts showed antimicrobial activity in tested MICs range. The highest antimicrobial activity showed the both extracts to Saccharomyces cerevisiae. The less antimicrobial effects had the both macromycetes extracts to Staphylococcus epidermis. Antimicrobial activity of macromycetes methanolic crude extracts to others tested microorganisms showed no effect or used concentration could be higher.

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Published

2023-09-05