ANALYSIS OF POLYMORPHISM OF CAST GENE AND CLPG GENE IN SHEEP BY PCR-RFLP METHOD
Keywords:
sheep, PCR-RFLP, calpastatin gene, callipyge geneAbstract
The work was oriented to identification of calpastatin gene polymorphism
and callipyge gene polymorphism and analysis of genotype structure in
population of sheep kept in Slovakia.
The material involved 96 sheep of breeds tsigai, improved valachian, east
friesian, lacaune and crossbreds lacaune and tsigai . Genomic DNA was
isolated by salting out method and used in order to estimate calpastatin and
callipyge genotypes by means of PCR-RFLP method. The PCR products were
digested with MspI restriction enzyme for calpastatin gene and FaqI
restriction enzyme for callipyge gene. In the total population of sheep was
detected homozygous genotype
MM – 0.87, heterozygous genotype MN – 0.13 for calpastatin gene.
Homozygous genotype NN has not been observed. For the callipyge gene was
detected homozygous genotype AA – 1.00 only. The heterozygous genotype
AG and homozygous genotype GG was not detected.
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