Detection of American Foulbrood in a Bee Family from Timiş County

Abstract

It seems that after information media lately have been detected more cases of American foulbrood in our country, but this is not yet known by the beekeepers. In November 2013 a beekeeper brought laboratory six dead bees and portions of honeycomb, because bees dying and he did not know why. Following clinical examination of dead bees was not obvious a major change so bacteriological examination was used. After taking over samples by a single dead bee in liquid culture medium, in the second day, the bacteriological examination emphasized a pure culture, rich of bacilli. There are rare situations when one can observe a pure culture of bacteria obtained from a single sample. After cultivation on special mediums, like agar with egg, the developing of rough colonies was no longer any doubt that the isolated bacteria are Paenibacillus larvae subsp. larvae (Bacillus larvae), etiologic agent for American foulbrood.