THE EVOLUTION OF SOME BIOCHEMICAL PARAMETERS IN THE HONEYBEES’ HAEMOLYMPH (A. M. CARPATHICA) COLLECTED IN THE INACTIVE SEASON
Keywords:
Apis mellifera carpathica honeybees, haemolymph, biochemical parametersAbstract
The biochemical analyses of the blood are largely used for the routine diagnosis
and especially for the metabolic survey in farm animals. These facts conduct us to
the idea that similar analyses, applied on honeybee hemolymph, could be used IN
monitoring the healthy state of honeybee colonies. The present studies represent
preliminary researches, which aimed to investigate the variability of the main
biochemical parameters in the hemolymph of the healthy honeybees (Apis
mellifera) in inactive season. The researches were carried out on honeybee
samples collected from 5 honeybee colonies belonging to a breeding apiary of the
Institute for Beekeeping Research and Development from Bucharest. In order to
perform the biochemical analyses, the honeybees samples, consisting in 50
individuals on sample (10 individuals/colony) were randomly collected and their
haemolimph collected, at different time intervals, in inactive season (fall-winter).
Totally, there were collected 250 haemolyph samples in a 2 years interval and the
following 21 biochemical parameters were analysed: GLU, HDL-c, ALP, T-cho,
Tprot, Alb., BUN, LDH, CPK,, Mg, IP), GGT, GOT, GPT, Ca, Cre,, Amy, T–BIL,
TG, UA.. The test was carried out after the collection and processing of the
samples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides
technique, respectively .During the 2nd part of the inactive season, the values of
most biochemical parameters increase in different proportions, their levels being
maintained also in the first part of the active seasons (April, May, June).The
values obtained for the main studied biochemical parameters in the haemolymph
of the healthy honeybees collected from honeybee colonies kept in natural
conditions show a highly variable evolution in the two consecutive years of
experiments during the inactive season.
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