Studies on the Antimicrobial Activity of Honey - A Review

Authors

  • Alexandru Nan University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului street, Timisoara - 300645, Romania
  • Roxana Popescu Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy of Timisoara, E. Murgu, 2, Timisoara, 300041 Romania
  • Marioara Nicoleta Caraba Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy of Timisoara, E. Murgu, 2, Timisoara, 300041 Romania
  • Liliana Petculescu Ciochina University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului street, Timisoara - 300645, Romania
  • Ioan Peț University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului street, Timisoara - 300645, Romania
  • Elena Peț University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului street, Timisoara - 300645, Romania
  • Daniela Pușcașiu Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy of Timisoara, E. Murgu, 2, Timisoara, 300041 Romania
  • Teodor Cerbulescu Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy of Timisoara, E. Murgu, 2, Timisoara, 300041 Romania
  • Mirela Ahmadi University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului street, Timisoara - 300645, Romania
  • Dorel Dronca University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului street, Timisoara - 300645, Romania
  • Gabi Dumitrescu University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului street, Timisoara - 300645, Romania

Keywords:

honey, antibacterial, antifungal, antiviral activity

Abstract

The biological activity of important honey, one of the most characteristics, which distinguishes it from other natural products and makes it important for the medical and therapeutic field. Its potential health benefits can be influenced both by its composition, which varies between different types of honey, and by the bioavailability of various phytochemical components and how they are absorbed and metabolized. It was found that a series of compounds contained in honey such as phenolic compounds, especially flavonoids, have an inhibitory effect against a wide spectrum of bacterial species, Gram-positive (G+) and Gram-negative (G-), but also an important  antifungal and antiviral action. These important properties of honey are due both to the quality of the polyphenols contained, which is more important than their quantity, but also to the high osmolarity, acidity, H2O2 content and the composition of non-peroxidic components.

References

Sharma, K., Sharma, K., Kumar, R., A review of physico-chemical and biological properties of honey, Journal of Entomology and Zoology Studies, 2024; 12(2): 153-161.

Jaganathan, S.K., Mandal, M. Antiproliferative effects of honey and of its polyphenols: a review. J Biomed Biotechnol, 2009, 1–13, doi:10.1155/2009/830616

Minden-Birkenmaier, B.A.; Cherukuri, K.; Smith, R.A.; Radic, M.Z.; Bowlin, G.L. Manuka honey modulates the inflammatory behavior of a dHL-60 neutrophil Model under the cytotoxic limit. Int. J. Biomater. 2019, 2019, 6132581.

Erejuwa, O.O.; Sulaiman, S.A.; AbWahab, M.S. Honey: A novel antioxidant. Molecules 2012, 17, 4400–4423.

Mustar, S.; Ibrahim, N. A Sweeter Pill to Swallow: A Review of Honey Bees and Honey as a Source of Probiotic and Prebiotic Products. Foods 2022, 11, 2102.

Fratianni, F.; Ombra, M.N.; d‘Acierno, A.; Caputo, L.; Amato, G.; De Feo, V.; Coppola, R.; Nazzaro, F. Polyphenols content and in vitro α-glycosidase activity of different Italian monofloral honeys, and their effect on selected pathogenic and probiotic bacteria. Microorganisms, 2021, 9, 1694.

Babaei, S.; Rahimi, S.; Torshizi, M.A.; Tahmasebi, G.; Miran, S.N. Effects of propolis, royal jelly, honey and bee pollen on growth performance and immune system of Japanese quails. Vet. Res. Forum, 2017, 7, 13–20.

Al-Mamary, M., Al-Meeri, A., Al-Habori, M. Antioxidant activities and total phenolics of different types of honey.Nutrition Research, 2002; 22(9):1041–1047

Al-Kafaween, M.A.; Hilmi, A.B.M.; Al-Jamal, H.A.N. The Beneficial Effects of Stingless Bee Kelulut Honey against Pseudomonas aeruginosa and Streptococcus pyogenes Planktonic and Biofilm: Doi. org/10.26538/tjnpr/v5i10. 15. Trop. J. Nat. Prod. Res. (TJNPR), 2021, 5, 1788–1796.

Mohammed, S.E.A.; Kabbashi, A.S.; Koko, W.S.; Ansari, M.J.; Adgaba, N.; Al-Ghamdi, A. In vitro activity of some natural honeys against Entamoeba histolytica and Giardia lamblia trophozoites. Saudi J. Biol. Sci. 2019, 26, 238–243.

Rani, G.N.; Budumuru, R.; Bandaru, N.R. Antimicrobial activity of honey with special reference to methicillin resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA). J. Clin. Diagn. Res., JCDR 2017, 11.

Morroni, G.; Alvarez-Suarez, J.M.; Brenciani, A.; Simoni, S.; Fioriti, S.; Pugnaloni, A.; Giampieri, F.; Mazzoni, L.; Gasparrini, M.; Marini, E. Comparison of the antimicrobial activities of four honeys from three countries (New Zealand, Cuba, and Kenya). Front. Microbiol., 2018, 9, 1378.

Mandal, M.D.; Mandal, S. Honey: Its medicinal property and antibacterial activity. Asian Pac. J. Trop. Biomed., 2011, 1, 154–160.

Johnston, M.; McBride, M.; Dahiya, D.; Owusu-Apenten, R.; Nigam, P.S. Antibacterial activity of Manuka honey and its components: An overview. AIMS Microbiol., 2018, 4, 655.

Goslinski, M.; Nowak, D.; Kł˛Ebukowska, L. Antioxidant properties and antimicrobial activity of manuka honey versus Polish honeys. J. Food Sci. Technol., 2020, 57, 1269–1277.

Girma, A.; Seo, W.; She, R.C. Antibacterial activity of varying UMF-graded Manuka honeys. PLoS ONE 2019, 14, e0224495

Feknous, N.; Boumendjel, M. Natural bioactive compounds of honey and their antimicrobial activity. Czech J. Food Sci., 2022, 40, 163–178.

Ahmadi–Motamayel, F.; Hendi, S.S.; Alikhani, M.Y.; Khamverdi, Z. Antibacterial activity of honey on cariogenic bacteria. J. Dent., 2013, 10, 10–15.

Albaridi, N.A. Antibacterial potency of honey. Int. J. Microbiol., 2019, 2464507.

Mohan, A.; Quek, S.-Y.; Gutierrez-Maddox, N.; Gao, Y.; Shu, Q. Effect of honey in improving the gut microbial balance. Food Qual. Saf., 2017, 1, 107–115.

Shahzad, A. In vitro antiviral activity of honey against varicella zoster virus (VZV): A translational medicine study for potential remedy for shingles. Transl. Biomed., 2012, 3, 2.

Obossou, E.K.; Shikamoto, Y.; Hoshino, Y.; Kohno, H.; Ishibasi, Y.; Kozasa, T.; Taguchi, M.; Sakakibara, I.; Tonooka, K.; Shinozuka, T. Effect of manuka honey on human immunodeficiency virus type 1 reverse transcriptase activity. Nat. Prod. Res. 2022, 36, 1552–1557.

Irish, J.; Carter, D.A.; Shokohi, T.; Blair, S.E. Honey has an antifungal effect against Candida species. Med. Mycol. 2006, 44, 289–291.

Moussa, A.; Noureddine, D.; Saad, A.; Abdelmelek, M.; Abdelkader, B. Antifungal activity of four honeys of different types from Algeria against pathogenic yeast: Candida albicans and Rhodotorula sp. Asian Pac. J. Trop. Biomed. 2012, 2, 554–557.

Guttentag, A.; Krishnakumar, K.; Cokcetin, N.; Hainsworth, S.; Harry, E.; Carter, D. Inhibition of dermatophyte fungi byAustralian jarrah honey. Pathogens, 2021, 10, 194.

Laxminarayan, R., Duse, A., Wattal, C., Zaidi, A.k., Wertheim, H.f., Sumpradit, N., Vlieghe, E., Hara, G.l., Gould, I.M., Goossens, H., Greko, C., SO, A.d., Bigdeli, M., Tomson, G., Woodhouse, W., Ombaka, E., Peralta, A.q., Qamar, F.N., Mir, F., Kariuki, S., Bhutta, Z.a., Coates, A., Bergstrom, R., Wright, G.D., Brown, E.D. &Cars, O. 2013. Antibiotic resistance-the need for global solutions. Lancet Infect Dis. 13(12): 1057-98.

Dixon B. Bacteria can't resist honey. Lancet Infect Dis., 2003;3:116.

Kwakman, P. H., te Velde, A. A., de Boer, L., Spejer, D., Vandenbroucke-Grauls, C. M., and Zaat, S. A., 2010. How honey kills bacteria. FASEB J., 24, 2576–2582.

Kwakman, P. H., and Zatt, S. A., 2012. Antibacterial components of honey. IUBMB Life 64, 48–55. doi: 10.102/iub.578.

Lusby P.E., Coombes A.L., Wilkinson J.M. Bactericidal Activity of Different Honeys against Pathogenic Bacteria. Archives of Medical Research., 2005;36(5):464–467.

Robson, V., Cooper, R. Using leptospermum honey to manage wounds impaired by radiotherapy: a case series. Ostomy Wound Manage, 2009; 55: 1, 38–47.

Ismail Z.B., Alshehabat M.A., Hananeh W., Daradka M., Ali J.H., El-Najjar E.K. Recent advances in topical wound healing products with special reference to honey: a review. Res. Opin. Anim. Vet. Sci., 2015;5(2):76–83.

Blair S.E., Cokcetin N.N., Harry E.J., Carter D.A. The unusual antibacterial activity of medical-grade Leptospermum honey: antibacterial spectrum, resistance and transcriptome analysis. Eur. J. Clin. Microbiol. Infect Dis., 2009;28:1199–1208.

Cooper R.A., Halas E., Molan P.C. The efficacy of honey in inhibiting strains of Pseudomonas aeruginosa from infected burns. J. Burn Care Rehabil., 2002;23:366–370.

Cooper R.A., Molan P.C., Harding K.G. The sensitivity to honey of Gram-positive cocci of clinical significance isolated from wounds. J. Appl. Microbiol., 2002;93:857–863.

French V.M., Cooper R.A., Molan P.C. The antibacterial activity of honey against coagulase-negative staphylococci. J. Antimicrob. Chemother., 2005; 56:228–231.

Price, J.N., Morgan, J.W., 2006. Variability of plant fitness influences range expansion of Leptospermum scoparium. Ecography, 29, 623–631.

Brady, N., Molan, P., Bang, L., 2004. A survey of non-manuka New Zealand honeys for antibacterial and antifungal activities. J. Apic., Res. 43, 47–52.

Julie, I., Shona, B., Carter, Dee A., 2011. The antibacterial activity of honey derived from Australian Flora. PLoS One 6 (3), e18229.

Mohd, I., Zainol, K., Mohd, Y., Mohd, Y., Mohd, Y., 2013. Antibacterial activity of selected Malaysian honey. BMC Complement Altern. Med., 13, 129.

Katrina, B., Calvin, S., 2014, Antibacterial Compounds of Canadian Honeys Target Bacterial Cell Wall Inducing Phenotype Changes, Growth Inhibition and Cell Lysis That Resemble Action of β-Lactam Antibiotics, PlosOne, https://doi.org/10.1371/journal.pone.0106967

Levites Y, Amit T, Youdim MB, Mandel S. Involvement of protein kinase C activation and cell survival/cell cycle genes in green tea polyphenol (-)-epigallocatechin 3-gallate neuroprotective action. J Biol Chem, 2002, 277: 30574-30580.

Singhal RL, Yeh YA, Praja N, Olah E, Sledge GW Jr, et al. Quercetin down-regulates signal transduction in human breast carcinoma cells. Biochemical and biophysical research communications, 1995, 208: 425-431.

Tripoli E, Guardia ML, Giammanco S, Majo DD, Giammanco ML. Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A review. Food Chemistry, 2007, 104: 466-479.

Chen S, Ding Y, Tao W, Zhang W, Liang T, et al. Naringenin inhibits TNF-α induced VSMC proliferation and migration via induction of HO-1. Food Chem Toxicol, 2012, 50: 3025-3031

Israili, Zafar, Antimicrobial Properties of Honey, American Journal of Therapeutics, 21(4): p 304-323, 2014.

Molan, P.C., Potential of honey in the treatment of wounds and burns, Am J Clin Dermatol, 2001; 2(1):13-9.

White J. W., Subers M. H., Schepartz A. I., 963. The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide, and its origin in a honey glucose-oxidase system. Biochem. Biophys. Acta., 73, 57–70

Ahed Abdulkhaliq & Khalid M. Swaileh, Physico-chemical properties of multi-floral honeyfrom the West Bank, Palestine, alestine, International Journal of Food Properties, 2017, 20:2, 447-454.

Atrouse, O.M., Oran, S.A., Al‐Abbadi, S.Y., 2004. Chemical analysis and identification of pollen grains from different Jordanian honey samples. International Journal of Food Science and Technology 39(4), 413-417.

Bucekova, M., Juricova, V., Monton, E., Martinotti, S., Ranzato, E., Majtan, J., 2018. Microwave processing of honey negatively affects honey antibacterial activity by inactivation of bee- derived glucose oxidase and defensin-1. Food Chemistry, 240, 1131-1136

Brudzynski, K.; Abubaker, K.; St. Martin, L.; Castle, A. Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. Front. Microbiol., 2011, 2, 213.

Carter, C., and Thornburg, R. W. , 2004. Is the nectar redox cycle a floral defense against microbial attack? Trends Plant Sci. 9, 320–324

Matzen, R.D., Leth-Espensen, J.Z., Jansson, T., Nielsen, D.S., Lund, M.N., Matzen, S., The Antibacterial Effect In Vitro of Honey Derived from Various Danish Flora, Dermatology Research and Practice, Volume 2018, Article ID 7021713, 10 pageshttps://doi.org/10.1155/2018/7021713

Brudzynski K., Lannigan R., 2012. Mechansim of honey bacteriostatic action against MRSA and VRE involves hydroxyl radicals generated from honey's hydrogen peroxide. Front. Microbiol., 3:36 10.3389/fmicb.2012.00036.

Estevinho, L., Pereira, A.P., Moreira, L., Dias, L.G, Pereira, E. Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food ChemToxicol, 2008.46: 3774–3779.

Pérez-Pérez, E., Vit, P., Huq, F., Flavonoids and polyphenols in studies of honey antioxidant activity, Int. J. Med. Plant Altern. Med., 2013, 1, 063-072.

Istasse Thibault, Nicolas Jacquet, Thomas Berchem, Eric Haubruge, Bach Kim Nguyen, Aurore Richel. Extraction of Honey Polyphenols: Method Development and Evidence of Cis Isomerization, Anal Chem Insights. 2016 Aug 10;11:49-57. doi: 10.4137/ACI.S39739. eCollection 2016.

Challacombe, CA., Abdel-Aal, E. M., Seetharamana, K., & Duizer, L. M. Influence of phenolic acid content on sensory perception of bread and crackers made from red or white wheat. Journal of Cereal Science, 2012, 56, 181–188.

Brudzynski, K, Sjaarda, C, & Maldonado-Alvarez, L. A new look on protein–polyphenol complexation during honey storage: Is this a random or organized event with the help of dirigent-like proteins? PLoS One, 2013, 8, 1–9.

Weston, R.J., 2000. The contribution of catalase and other natural products to the antibacterial activity of honey: a review, Food Chemistry, 71(2): 235-239.

Aljadi, A.M., Yusoff, K.M., 2003. Isolation and Identification of Phenolic Acids in Malaysian Honey with Antibacterial Properties, Turkish Journal of Medical Sciences, 33(4).

Mazol, I., Sroka, Z., Sowa, A., Ostrowska, A., Drys, A., Gamian, A., 2016. Antiradical and antimicrobial activity of phenolic fractions obtained from honeys, Acta Pol Pharm., 73: 379-388.

Szweda, P., 2017. Antimicrobial Activity of Honey. In: De Alencar Arnaut De Toledo, V., Ed., Honey Analysis, Vol. 1, IntechOpen, London, 215-232.

Mundo, M.A., Padilla-Zakour, O.I., Worobo,R.W., 2004. Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys, International Journal of Food Microbiology, 97(1): 1-8.

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Published

2024-10-31