Microbial Quality of Smoked Cow Cheese “Parenica”
Keywords:
cow milk cheese, “parenica”, microorganisms, MALDI-TOF MS BiotyperAbstract
Slovak smoked cow cheese “Parenica” is a traditional dairy product with a protected geographical indication. An amount of 96 samples were studied in the present study. The samples were examined for coliforms (VRBL medium at 37 °C for 24-48 h), for lactic acid bacteria (on MRS media at 30 °C for 48-72 h), microscopic filamentous fungi (MEA at 25 °C for 5 days) and total microbial counts (PCA at 30 °C for 48-72 h). Individual species of microorganisms isolated from smoked cheese “parenica” were identified with MALDI-TOF MS Biotyper. Totally, 41 species of microorganisms were identified. The most abundant species were Lactobacillus fermentum, Lactococcus lactis, Enterococcus faecium, and Escherichia coli. Microscopic filamentous fungi were represented by the genus Rhizopus spp. and yeasts by species of Yarrowia lipolytica and Kluyveromyces lactis. Of the total number of isolates 47.66% were lactic acid bacteria, 27.84% coliform bacteria, 18.66% yeasts and 5.85% of microscopic filamentous fungi. The occurrence of microorganisms increased in the summer then the highest values of coliform bacteria were detected 1.74 log cfu/g. The lactic acid bacteria counts found in summer were 2.19 log cfu/g, and the highest counts were observed in December - 2.66 log cfu/g.
References
Garofalo, C., Osimani, A., Milanović, V., Aquilanti, L., De Filippis, F., Stellato, G., Di Mauro, S., Turchetti, B., Buzzini, P., Ercolini, D., Clementi, F., Bacteria and yeast microbiota in milk kefir grains from different Italian regions, Food microbiology, 2015, 49, (4), 123-133.
De Angelis, M., Gobbetti, M., Pasta Filata Chesses: Traditional Pasta Filata Cheese, 2016, 83(21), 4-17.
Zimanová, M., Greifová, M., Body, P., Herian, K., Technology of steamed cheese production, Chemické listy, 2016, 110(4), 259-260.
Ledenbach, H. L., Marshall, R. T., Microbiological spoilage of dairy products. In: W. H. Sperber, M. P. Doyle (eds), Compendium of the microbiological spoilage of foods and beverages, food microbiology and food safety, 2009, Springer, New York.
Kongo, M., Malcata, F. X. Cheese: In Chemistry and Microbiology, from Encyclopedia of Food and Health. Elsevier Science & Technology, 2015, 1(1), pp. 735-740.
Raposo, A., P ́erez, E., Tinoco de Faria, C., Ferr ́us, M. A., Carras-cosa, C., Food spoilage by Pseudomonas spp. – An overview. In Om. V.Singh (ed.), Foodborne pathogens and antibiotic resistance. John Wiley& Sons, Inc. Published, Hoboken.
Lu, M., Wang, N. S. Spoilage of milk and dairy products. In A. Bevilac-qua, M. R. Corbo, M. Sinigaglia (eds), The microbiological quality of food. Woodhead Publishing, 2017, Amsterdam.
Kačániová, M., Felšöciová, S., Haščík, P., Nagyová, Ľ., Horská, E., Terentjeva, M., Kunová, S., Diversity of fungi in the traditional slovak cheese "Parenica", Lucrări ştiinţifice. Zootehnie şi biotehnologii, 2019, 52(2), 94-99.
Kačániová, M., Felšöciová, S., Haščík, P., Nagyová, Ľ., Horská, E., Godočiková, L., Lopašovský, Ľ., Terentjeva, M., Kunová, S, Lactic acid bacteria isolated from traditional cheese, Lucrări ştiinţifice. Zootehnie şi biotehnologii, 2019, 52(2), 83-88.
Cardoso, V. M., Borelli, B. M., Lara, C. A., Soares, M. A., Pataro, C., Bodevan, E. C., Rosa, C. A., The influence of seasons and ripening time on yeast communities of a traditional Brazilian cheese, Food Research International, 2015, 69(2), 331-340.
Kačániová, M., Kunová, S., Nagyová, Ľ., Horská, E., Haščík, P., Terentjeva, M., Application of MALDI-TOF mass spectrometry for identification of bacteria isolated from traditional Slovak cheese “Parenica”, Journal of Microbiology, Biotechnology and Food Sciences, 2019, 8(6), 1294-1297.
Kačániová, M., Terentjeva, M., Kunová, S., Nagyová, Ľ., Horská, E., Haščík, P., Kluz, M., Puchalski, C., Identification of the Slovak traditional cheese "Parenica" microflora. Acta agraria Debreceniensis, 2018, 150, 227-239.
Kačániová, M., Terentjeva, M., Kunová, S., Nagyová, Ľ., Horská, E., Haščík, P., Kluz, M., Puchalski, C., Microorganisms isolated from the Slovak traditional cheese "Parenica" and their identification with mass spectrometry, Lucrări ştiinţifice. Zootehnie şi biotehnologii, 2018, 51(2), 76-82.
Kačániová, M., Kunová, S., Horská, E., Nagyová, Ľ., Puchalski, C., Haščík, P., Terentjeva, M., Diversity of microorganisms in the traditional Slovak cheese., Potravinárstvo Slovak Journal of Food Sciences, 2019, 13(1), 532-537.
Kačániová, M., Nagyová, Ľ., Štefániková, J., Felšöciová, S., Godočiková, L., Haščík, P., Horská, E., Kunová, S., The characteristic of sheep cheese ”Bryndza” from different regions of Slovakia based on microbiological quality, Potravinárstvo Slovak Journal of Food Sciences, 2020, 14(1), 69-75.
Kirmaci, H., Özer, B. H., Akçelik, M., Akçelik, N., Identification and characterisation of lactic acid bacteria isolated from traditional Urfa cheese, International Journal of Dairy technology, 2015, 69(2), 301-307.
Tabla, R., Gómez, A., Simancas, A., Rebollo, J. E., Molina, F., Roa, I., 2016. Enterobacteriaceae species during manufacturing and ripening of semi–hard and soft raw ewe’s milk cheese, Small Ruminant Research, 2016, 145, 123-129.
Trmcic, A., Chauhan, K., Kent, D. J., Ralyea, R. D., Martin, N. H., Boor, K. J., Wiedmann, M., Coliform detection in cheese is associated with specific cheese characteristics, but no association was found with pathogen detection, Journal of Dairy Science, 2016, 99(8), 6105-6120.
