Human Adrenocarcinoma (H295R) Cells as Potential Predictors of Bisphenols Ability to Interfere with Steroid Hormone Production: A Mini Review
Keywords:
bisphenols, endocrine disruptors, H295R, steroidogenesisAbstract
The focus on the refinement, reduction and replacement of animal use in toxicity testing requires the development of cell-based systems that mimic the effects of xenobiotics including bisphenols in human tissues. The human H295R adreno-carcinoma cell line provides a good in vitro system for the analysis of the human adrenal steroidogenic pathway at the level of hormone production and gene expression, because it expresses genes that encode for all the key enzymes for steroidogenesis. In this review we provide an introduction to H295R cell line and its use for research of toxicological effects of bisphenols, which represents an important contaminant.
References
Hecker, M., Giesy, P. J., Novel trends in endocrine disruptor testing: the H295R Steroidogenesis Assay for identification of inducers and inhibitors of hormone production. Anal Bioanal Chem. 2007, doi: 10.1007/s00216-007-1657-5
Haggard, D. E., Karmaus, A. L., Martin, M. T., Judson, R. S., Setzer, R. W., & Paul Friedman, K., High-Throughput H295R Steroidogenesis Assay: Utility as an Alternative and a Statistical Approach to Characterize Effects on Steroidogenesis. Toxicological Sciences. 2017. doi: 10.1093/toxsci/kfx274
Maglich, J. M., Kuhn, M., Chapin, R. E., & Pletcher, M. T., More than just hormones: H295R cells as predictors of reproductive toxicity. Reproductive Toxicology. 2014. doi: 10.1016/j.reprotox.2013.12.009
Chen, M.Y., Ike, M., Fujita, M., Acute toxicity, mutagenicity, and estrogenicity of bisphenol-A and other bisphenols, Environ. Toxicol., 2002, 17, 80-86
Delfosse, C.V., Grimaldi, M., Pons, J., Boulahtouf, A., Maire, A., Cavailles, V., Labesse, G., Bourguet, W., Balaguer, P., Structural and mechanistic insights into bisphenols action provide guidelines for risk assessment and discovery of bisphenol A substitutes, Proc. Natl. Acad. Sci. U. S. A., 2012, 109, 14930-14935
Hilscherova, K., Jones, P.D., Gracia, T., Newsted, J.L., Zhang, X., Sanderson, J.T., Yu, R.M., Wu, R.S., Giesy, J.P., Assessment of the effects of chemicals on the expression of ten steroidogenicgenes in the H295R cell line using real-time PCR, Toxicol. Sci. 81, 78-89
Sanderson J. T., Seinen W., Giesy J. P., Van Den Berg M., 2-Chloro-s-triazine herbicides induce aromatase (CYP19) activity in H295R human adrenocortical carcinoma cells: A novel mechanism for estrogenicity?, Toxicol. Sci. 54, 121–127
OECD Guideline for the testing of chemicals H295R Steroidogenesis Assay, 2011. Home page address: https://ntp.niehs.nih. gov/iccvam/suppdocs/feddocs/oecd/oecd-tg456-2011-508.pdf
Gazdar, A.F., Oie, H.K., Shackleton, C.H., Chen, T.R., Triche, T.J., Myers, C.E., Chrousos, G.P., Brennan, M.F., Stein, C.A., La Rocca, R.V., Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res. 1990, 50, 5488-5496
Gracia, T., Hilscherova, K., Jones, P.D., Newsted, J.L., Higley, E.B., Zhang, X., Hecker, M., Murphy, M.B., Yu, R.M., Lam, P.K., Wu, R.S., Giesy, J.P., Modulation of steroidogenic gene expression and hormone production of H295R cells by pharmaceuticals and other environmentally active compounds. Toxicol. Appl. Pharmacol. 2007, 225, 142-153
Breen, M.S., Breen, M., Terasaki, N., Yamazaki, R.B, Conolly Computational model of steroidogenesis in human H295R cells to predict biochemical response to endocrine-active chemicals: model development for metyrapone. Environ. Health. Perspect., 2010, 118, 265-272
Close, D.M., Rotroff, D.J., Dix, K.A., Houck, T.B., Knudsen, M.T., Martin, K.W., McLaurin, D.M., Reif, K.M., Crofton, A.V., Singh, M., Xia, R., Huang, R.S., Judson Using in vitro high throughput screening assays to identify potential endocrine-disrupting chemicals, Environ. Health. Perspect., 2013, 121, 7-14
Feng, Y., Jiao, Z., Shi, J., Li, M., Guo, Q., & Shao, B., Effects of bisphenol analogues on steroidogenic gene expression and hormone synthesis in H295R cells. Chemosphere, 2016, 147, 9-19. Doi :10.1016/j. chemosphere.2015.12.081
Zhang, X., Chang, H., Wiseman, S., He, Y., Higley, E., Jones, P., Wong, Ch., Al-Khedhairy, A., Giesy, J., Hecker, M., Bisphenol A Disrupts Steroidogenesis in Human H295R Cells. Toxicological Sciences, 2011, 121 (2), 320-327. doi: 10.1093/toxsci/kfr061
Ji, K., Hong, S., Kho, Y. Choi, K., Effects of bisphenol S exposure on endocrine functions and reproduction of zebrafish Environ., Sci. Technol., 2013, 47, 8793-8800
Naderi, M., Wong, M.Y., Gholami, F., Developmental exposure of zebrafish (Danio rerio) to bisphenol-S impairs subsequent reproduction potential and hormonal balance in adults, Aquat. Toxicol., 2014, 148, 195-203
Kinmer, I., Bisphenol A and immunotoxic potential: A commentary, Regul Toxicol Pharmacol., 2017, 90: 358-363. doi: 10.1016/j.yrtph.2017.08.022
Moral, R., Wang, R., Russo, I.H., Lamartiniere, C.A., Pereira, J., Russo, J., Effect of prenatal exposure to the endocrine disruptor bisphenol A on mammary gland morphology and gene expression signature, J. Endocrinol., 2008, 196, 101-112
Miyawaki, J., Sakayama, K., Kato, H. Yamamoto, H., Masuno, H., Perinatal and postnatal exposure to bisphenol A increases adipose tissue mass and serum cholesterol level in mice, J. Atheroscler. Thromb., 2007, 14, 245-252
Stojanoska, M.M., Milosevic, N., Milic, N., Abenavoli, L., The influence of phthalates and bisphenol A on the obesity development and glucose metabolism disorders, Endocrine., 2017, 5 (3), 666-681, doi: 10.1007/s12020-016-1158-4
Nielsen, F. K., Hansen, C. H., Fey, J. A., Hansen, M., Jacobsen, N. W., Halling-Sørensen, B., Styrishave, B., H295R cells as a model for steroidogenic disruption: A broader perspective using simultaneous chemical analysis of 7 key steroid hormones. Toxicology in Vitro, 2012, 26 (2), 343–350. doi: 10.1016/j.tiv.2011.12.008
Crain, D.A., Janssen, S.J., Edwards, T.M., Heindel, J., Ho, S.M., Hunt, P., Iguchi, T., Juul, A., McLachlan, J.A., Schwartz, J., Skakkebaek, N., Soto, A.M., Swan, S., Walker, C., Woodruff, T.K., Woodruff, T.J., Giudice, L.C., Guillette L.J., Female reproductive disorders: the roles of endocrine-disrupting compounds and developmental timing, Fertil. Steril., 2008, 90 (4), 911-940
Melzer, D., Rice E.N., Lewis, C., Henley E.W., Galloway, S.T., Association of urinary bisphenol a concentration with heart disease: evidence from NHANES 2003/06, 2010, 5 (1), doi: 10.1371/journal. pone.0008673
Vandenberg, L.N., Colborn, T., Hayes, T.B., Heindel, J.J., Jacobs Jr., D.R., Lee, D.H., Shioda, T., Soto, A.M., Vom Saal, F.S., Welshons, W.V., Zoeller, R.T., Myers J.P., Hormones and endocrine-disrupting chemicals: low-dose effects and nonmonotonic dose responses Endocr. Rev., 2012, 33, 378-455
Rochester J.R., Bisphenol A and human health: a review of the literature Reprod. Toxicol., 2013, 42, 132-155
Rezg, R., El-Fazaa, S., Gharbi, N., Mornagui B., Bisphenol A and human chronic diseases: current evidences, possible mechanisms, and future perspectives Environ. Int., 2014, 64, 83-90
Rancière, F., Lyons, J.G., Loh, V.H., et al., Bisphenol A and the risk of cardiometabolic disorders: a systematic review with meta-analysis of the epidemiological evidence, Environ Health., 2015, 14-46. doi: 10.1186/s12940-015-0036-5
Clark E., Sulfolane and sulfones Kirk-othmer Encyclopedia of Chemical Technology, John Wiley & Sons, New York, NY, 2012
Grumetto, L., Montesano, D., Seccia, S., Albrizio, S., Barbato, F., Determination of bisphenol a and bisphenol B residues in canned peeled tomatoes by reversed-phase liquid chromatography, J Agric Food Chem., 2008, 56 (22), 10633-10637. doi: 10.1021/ jf802297z
Fiege, H., Voges, H. W., Hamamoto, T., Umemura, S., Iwata, T., Miki, H., et al., Phenol derivatives Ullmann's Encyclopedia of Industrial Chemistry, Wiley-VCH Verlag GmbH & Co. KGaA, Winheim, 2000
Cabaton, N., Chagnon, M.C., Lhuguenot, J.C., Cravedi, J.P., Zalko, D., Disposition and metabolic profiling of bisphenol F in pregnant and nonpregnant rats, Journal of Agricultural and Food Chemistry, 2006, 54 (26), 10307–10314.
National Toxicology Program Chemical Information Profile for Bisphenol AF [CAS No. 1478-61-1]: Supporting Nomination for Toxicological Evaluation by the National Toxicology Program, 2008
Liao, C., Kannan K., Concentrations and profiles of bisphenol A and other bisphenol analogues in foodstuffs from the United States and their implications for human exposure, J. Agric. Food. Chem., 2013, 61, 4655-4662
Liao, C., Kannan K., A survey of alkylphenols, bisphenols, and triclosan in personal care products from china and the United States, Arch. Environ. Contam. Toxicol., 2014, 7, 50-59
Liao, C., Liu, F., Kannan K., Bisphenol s, a new bisphenol analogue, in paper products and currency bills and its association with bisphenol a residues, Environ. Sci. Technol., 2012, 46, 6515-6522
Song, S., Song, M., Zeng, L., Wang, T., Liu, R., Ruan, T., Jiang G., Occurrence and profiles of bisphenol analogues in municipal sewage sludge in China Environ. Pollut., 2014, 186, 14-19
Yang, X., Liu, Y., Li, J., Chen, M., Peng, D., Liang, Y., Song, M., Zhang, J., Jiang G., Exposure to bisphenol AF disrupts sex hormone levels and vitellogenin expression in zebrafish, Environ. Toxicol., 2014, doi: 10.1002/tox.22043
Yang, Y., Guan, J., Yin, J., Shao, B., Li, H., Urinary levels of bisphenol analogues in residents living near a manufacturing plant in south China, Chemosphere, 2014, 112, 481-486
Yang, Y., Lu, L., Zhang, J., Yang, Y., Wu, Y., Shao, B., Simultaneous determination of seven bisphenols in environmental water and solid samples by liquid chromatography-electrospray tandem mass spectrometry, J. Chromatogr. A, 2014, 1328, 26-34.
