Comparative Assessing of Effects of some Active Compounds from Natural Sources on Lead Antagonism to Manganese in Carassius gibelio Bloch
Keywords:
fish, lead intoxication, lyophilized algae, lyophilized herbs, manganeseAbstract
The purpose of this paper was to assess comparatively the manganse tissue distribution in selected organs of Prussian carp specimens before and after experimental lead intoxication, with and without some lyophilized herbs and algae addition in fish diet.
The study involved 180 individuals with similar biometric measurements, caught from a local fish farm and equally divided in four experimental groups, as follows: C group (without treatment), E1 group (75 ppm Pb into water as Pb(NO3)2 x ½H2O), E2 group (75 ppm Pb into water+2% lyophilized garlic in feed), E3 group (75 ppm Pb into water+2% lyophilized cilantro in feed), E4 group (75 ppm Pb into water+2% lyophilized chlorella in feed), E5 group (75 ppm Pb into water+2% lyophilized spirulina in feed).
Tissue samples (gills, muscle, heart, skin and scales, intestine, liver, brain, gonads, kidney) were collected and processed at the end of a 21 days experimental period.
Atomic absorption measurements of the analytes were carried out with a AAS-VARIAN model.
Determination of position and variation parameters was performed using SPSS software. Differences between groups were tested based on the Friedman test using also SPSS, Nonparametric tests/K Related Samples.
The manganese tissue concentrations were significantly reduced by sublethal lead-induced poisoning (p<0.001). But spirulina lyophilized closely followed by the garlic lyophilized were able to attenuate much of the lead antagonistic effect with respect to manganese transport and tissue uptake. In the renal tissue, spirulina lyophilized, elevates the Mn at the level of the control group. Garlic instead is most effective in liver and intestinal tissue.
References
Flora, G., Gupta, D. Tiwari, A., Toxicity of lead: A review with recent updates, Interdiscip Toxicol. 2012, 5(2), 47–58.
Ashmead, H. D., The Roles of Amino Acid Chelates in Animal Nutrition. Westwood: Noyes Publications, 1993.
Hussain Al-Gubory, K., Nutritional Antioxidant Therapies: Treatments and Perspectives, Chapter 1. Phytochemicals, antioxidant therapy, opportunities, and challenges, 2017, Springer Edition, pp 1-26.
Uchikawa, T., Kumamoto, Y., Maruyama, I., Kumamoto, S., Ando, Y., Yasutake, A., J. Toxicol. Sci., 2011, 36(1), 121.
Abdalla, F. H., Bellé, L. P., De Bona, K. S., Bitencourt, E. R., Pigatto, A. S. M., Moretto, B., Food and Chemical Toxicology, 2010, 48(1), 417.
Deldar, K., Nazemi, E., Balali-Mood, M., Journal of Birjand University of Medical Sciences, 2008, 15, 11.
Nicula, M., Pacala, N., Stef, L., Pet I., Radulov, I., Iancu, T., Ahmadi, M., Dronca, D., Gherbon, A., Synergic and Antagonistic Action of Active Principles on Chromium-Lead Antagonism - in vivo Experiments, Rev.Chim., 2019, 70(2), 455-458.
Nicula, M., Pacala, N., Stef, L., Pet, I. Bencsik, I. Radulov I., Iancu, T., Tulcan, C., Dragomirescu, M., Dronca, D., Berbecea, A., Dumitrescu, G., Simiz E., Ahmadi, M., Marcu, A., The Effect of Active Principles of Cilantro and Spirulina Powder on Lead Antagonism to Zinc and Iron in Carassius gibelio, Animal Science and Biotechnologies, 2017, 50(1), 265- 271
Nicula, M., Pacala, N., Stef, L., Pet, I., Bencsik, I., Iancu, T., Tulcan, C., Dragomirescu, M., Dronca, D., Berbecea, A., Simiz, E., Ahmadi, M., Marcu, A., Erina, S., Ameliorative Effect of Active Principles from Cilantro and Spirulina Powder on Lead Antagonism to Calcium and Magnesium in Carassius gibelio Bloch
Animal Science and Biotechnologies, 2018, 51 (2), 125-129.
Phys Org, Researchers put manganese's role in coastal waters under the microscope, 2016, https://phys.org/news/2016-06-manganese-role-coastal-microscope.html
Chanda, S., Paul B. N., Ghosh, K., Giri, S. S., Dietary essentiality of trace minerals in aquaculture-A Review, Agri. Review, 2015, 36(2), 100-112).
Fish Feed Technology Chapter 7. The Minerals
http://www.fao.org/3/x5738e/x5738e00.htm#Contents.
Chemical properties of manganese - Health effects of manganese - Environmental effects of manganese
https://www.lenntech.com/periodic/elements/mn.html
Watts, D. L., Nutrient Interrelationships Minerals-Vitamins-Endocrines, Orthomolecular Medicine, 1990, 5(1).
