Staphylococcus spp. Isolated from Spiders and their Antimicrobial Sensitivity against Essential Oils


  • Miroslava Kačániová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia
  • Mariia Fedoriak Yuriy Fedkovych Chernivtsi National University, Kotsubynskogo, 2, 58012 Chernivtsi, Ukraine
  • Margarita Terentjeva Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, Jelgava, LV-3004, K. Helmaņa iela 8, Latvia
  • Maciej Kluz University of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, Poland
  • Pawel Hanus University of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, Poland
  • Czeslaw Puchalski University of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, Poland


Staphylococcus, antimicrobial activity, spiders


Research on insects and their bacterial communities provides valuable information on potential interaction between the host and their symbiotic bacteria. The aim of the present study was to evaluate antimicrobial activity of essential oils against Staphylococcus spp. isolated from spiders. Spiders samples were investigated microbiologically and Staphylococcus isolates were confirmed with MALDI TOF MS Biotyper. Antimicrobial activity of Satureja hortensis L., Pimpinella anisum L., Rosmarinus officinalis L., Salvia officinalis L. and Ocimum basilicum L. essential oils was detected.  Altogether five Staphylococcus species were identified: S. capitis, S. epidermidis, S. haemolyticus, S. warneri and S. xylosus. All bacterial strains were resistant of ciprofloxacin, levofloxacin, amikacin and gentamicin. S. haemoliticus was the most resistant against essential oils of Rosmarinus officinalis L., Salvia officinalis L. S. warneri and S. xylosus were the most sensitive against antimicrobial action of the applied EOs.


Baxtrom, C., Mongkolpradit, T., Kasimos, J.N., Braune, L.M., Wise, R.D., Common house spiders are not likely vectors of community-acquired methicillin-resistant Staphylococcus aureus infections, Journal of Medical Entomology 2006, 43, 962-965

Gaver-Wainwright, M.M., Zack, R.S., Foradori, M.J., Lavine, L.C., Misdiagnosis of spider bites: bacterial associates, mechanical pathogen transfer, and hemolytic potential of venom from the Hobo spider, Tegenaria agrestis (Araneae: Agelenidae), Journal of Medical Entomology 2011, 48, 382-388

Mascarelli, P.E., Maggi, R.G., Hopkins, S., Mozayeni, B.R., Trull, C.L., Bradley, J.M., Hegarty, B.C., Breitscgwerdt, E.B., Bartonella henselae infection in a family experiencing neurological and neurocognitive abnormalities after woodlouse hunter ,s pider bites, Parasites and Vectors 2013, 6, 98

Silvestre, F.G., de Castro, C.S., de Moura, J.F., Giusta, M.S., De Maria, M., Alvares, E.S.S., Lobato, F.C.F. Assis, R.A., Goncalves, L.A., Gubert, I.C., Chavez-Olortegui, C., Kalapothakis, E. Characterization of the venom from the Brazilian Brown Spider Loxosceles similis Moenkhaus, 1898 (Araneae, Sicariidae). Toxicon, 2005, 46: 927–936.

Monteiro, C.L.B., Rubel, R., Cogo, L.L., Mangili, O.C., Gremski, W., Isolation and identification of Clostridium perfringens in the venom and fangs of Loxosceles intermedia (brown spider): enhancement of the dermonecrotic lesion in loxoscelism, Toxicon, 2002, 40, 409-418

Bili, M., Cortesero, A.M., Mougel, C., Gauthier, J.P., Ermel, G., Simon, J.C., Outreman, Y., Terrat, S., Mahéo, F., Poinsot, D., Bacterial community diversity harboured by interacting species, PLoS One, 2016

Monteiro, C.C., Villegas, L.E.M., Campolina, T.B., Pires, A.C., Miranda, J.C., Pimenta, P.F., Secundino, N.F. Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing, Parasit Vectors, 2016, 9, 480

Fryxell, R.T.T., DeBruyn, J.M. The microbiome of Ehrlichia-infected and uninfected lone star ticks (Amblyomma americanum), PLoS One, 2016

Gotoh, T., Noda, H., Ito, S., Cardinium symbionts cause cytoplasmic incompatibility in spider mites Heredity 2007, 98, 13-20

Brownlie, J.C., Cass, B.N., Riegler, M., Witsenburg, J., Iturbe-Ormaetxe, I., McGraw, E.A., O’Neill, S.L., Evidence for metabolic provisioning by a common invertebrate endosymbiont, Wolbachia pipientis, during periods of nutritional stress, PLoS Pathogens, 2009

Himler, A.G., Adachi-Hagimori, T., Bergen, J.E., Kozuch, A., Kelly, S.E., Tabashnik, B.E., Chiel, E., Duckworth, V.E., Dennehy, T.J., Zchori-Fein, E., Hunter, M.S. Rapid spread of a bacterial symbiont in an invasive whitefly is driven by fitness benefits and female bias, Science, 2011, 332, 254-256

Goodacre, S.L., Martin, O.Y., Thomas, C.F.G., Hewitt, G.M. Wolbachia and other endosymbiont infections in spiders, Molecular Ecology, 2006, 15, 517-527

Duron, O., Hurst, G.D.D., Hornett, E.A., Josling, J.A., Engelstädter, J., High incidence of the maternally inherited bacterium Cardinium in spiders, Molecular Ecology, 2008, 17, 1427-1437

Martin, O.Y., Goodacre, S.L., Widespread infections by the bacterial endosymbiont Cardinium in arachnids, Journal of Arachnology, 2009, 37, 106-108

Otto, M., Staphylococcus colonization of the skin and antimicrobial peptides, Expert review of dermatology, 2010, 5(2), 183-195

Ugur, A., Varol, Ö., Ceylan, Ö., Antibacterial Activity of Sideritis curvidens and Sideritis lanata from Turkey, Pharmaceutical Biology, 2005, 43(1), 47-52

Alhaj, N., Shamsudin, M., Zamri, H., Abdullah, R, Extraction of essential oil from Nigella sativa using supercritic al carbon dioxide: study of antibacterial activity, American Journal of Pharmacology and Toxicology, 2008, 3(4), 225-228

Fisher, K., Phillips, C., The mechanism of action of a citrus oil blend against Enterococcus faecium and Enterococcus faecalis, Journal of Applied Microbiology, 2009, 106(4), 1343-1349

European Committee on Antimicrobial Susceptibility Testing (EUCAST). Antimicrobial susceptibility testing: Eucast disk diffusion method, version 3.0 from April 2013

European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters, version 5.0 valid from 2015-01-01

Kačániová, M., Terentjeva, M., Vukovic, N., Puchalski, C., Roychoudhury, S., Kunová, S., Kluga A., Tokár, M., Kluz, M., Ivanišová, E., The antioxidant and antimicrobial activity of essential oils against Pseudomonas spp. isolated from fish, Saudi Pharmaceutical Journal, 2017, 25(8), 1108-1116

Ahlstrand, K., Svensson, L., Persson, U., Tidefelt, B.S., Glycopeptide resistance in coagulase-negative staphylococci isolated in blood cultures from patients with hematological malignancies during three decades,

European Journal of Clinical Microbiology and Infection Disease, 2011, 30, 1349-1354

Mirrett, S., Weinstein, M.P., Reimer, L.G., Wilson, M.L., Reller L.B., Relevance of the number of positive bottles in determining clinical significance of coagulase-negative staphylococci in blood cultures, Journal of Clinical Microbiology, 2001, 39,3279-3281

Drozenova, J., Petras P., Characteristics of coagulase-negative staphylococci isolated from hemocultures, Epidemiology Mikrobiology Imunology, 2000, 49, 51-58

Huang, S.Y. Tang, R.N. Chen, S.Y., Chung, R.L., Coagulase-negative staphylococcal bacteremia in critically ill children: risk factors and antimicrobial susceptibility, Journal of Microbiology and Immunology Infect, 2003, 36, 51-55

Jain, A., Agarwa, J., Bansal, S., Prevalence of methicillin-resistant, coagulase-negative staphylococci in neonatal intensive care units: findings from a tertiary care hospital in India, Journal of Medicinal Microbiology, 2004, 53, 941-944

Knauer, A., Fladerer, P., Strempfl C., Effect of hospitalization and antimicrobial therapy on bantimicrobial resistance of colonizing Staphylococcus epidermidis, Wien Klin Wochenschr, 2004, 116, 489-494

Lv, F., Lianga, H., Yuan, Q., Li, C., In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms, Food Research International, 2011, 44, 3057-3064

Goni, P., Lopez, P., Sanchez, C., Gomez-Lus, R., Becerril, R., Nerın, C., Antimicrobial activity in the vapour phase of a combination of cinnamon and clove essential oils, Food Chemistry, 2009, 116, 982-989

Fu, Y., Zu, Y., Chen, L., Shi, X., Wang, Z., Sun, S., Antimicrobial activity of clove and rosemary essential oils alone and in combination, Phytotherapy Research,

, 21, 989-994

Al-Bayati, F.A., Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts, Journal of Ethnopharmacology, 2008, 116, 403-406

Jarrar, N., Abu-Hijleh, A.A., Adwan, K., Antibacterial activity of Rosmarinus officinalis

L. alone and in combination with cefuroxime against methicillin – resistant Staphylococcus aureus

Asian Pacific Journal of Tropical Medicine (2010), 121-123

Akhtar, A., Deshmukh, A.A., Bhonsle, A.V., Kshirsagar, P.M., Koleka, M.A., In vitro antibacterial activity of Pimpinella anisum fruit extracts against some pathogenic bacteria Veterinary World, 2008, 1(9), 272-274

Boyraz, N., Ozcan M., Inhibition of phytopathogenic fungi by essential oil, hydrosol, ground material and extract of summer savory (Satureja hortensis L.) growing wild in Turkey, International Journal of Food Microbiology, 2006, 107, 238-242

Adiguzel, A., Ozer, H., Kilic, H., Cetin, B., Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi, Czech Journal of Food Science, 2007, 25(2), 81-89

Razzaghi-Abyaneh, M., Shams-Ghahfarokhi, M., Yoshinari, T., Rezaee, M.B., Jaimand, K., Nagasawa, H., Sakuda, S., Inhibitory effects of Satureja hortensis L. essential oil on growth and aflatoxin production by Aspergillus parasiticus, International Journal of Food Microbiology, 2008, 123, 228-233

Omidbeygi, M., Barzegar, M., Hamidi, Z., Naghdibadi, H., Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste, Food Control, 2006, 18, 1518-1523

Dikbas, N., Kotan, R., Dadasoglu, F., Sahin, F., Control of Aspergillus flavus with essential oil and methanol extract of Satureja hortensis. International Journal of Food Microbiology, 2008, 124, 179–182.

Bosni, T., Softi, D., Gruji-Vasi, J., Antimicrobial activity of some essential oils and major constituents of essential oils, Acta Medica Botanica, 2006, 35(1), 9-14

Skovic, M., Tzakaou, O., Pitarokili, D., Couladis, M., Antifungal activities of selected aromatic plants growing wild in Greece, Nahrung,, 2002, 46(5), 317-320

Baratta, M.T., Dorman, H.J.D., Deans, S.G., Chemical composition, antimicrobial and antioxidative activity of laurel, sage, rosemary, oregano and coriander essential oils, Journal of Essential Oil Research, 1998, 10, 618-627

Miti-Ulafi, D., Vukovi-Gai, B., Kneevi-Vukevi, J., Stankovi, S., Simi, D., Comparative study on the antibacterial activity of volatiles from sage (Salvia officinalis L.)”, Archives of Biological Sciences,Belgrade, 2005, 57(3), 173-178.